Generation of conditional knockout alleles for PRL-3
Hong Yan
Dong Kong
Xiaomei Ge
Xiang Gao
Xiao Han
摘要：Phosphatase of regenerating liver-3 (PRL-3) is a member of the protein tyrosine phosphatase (PTP) superfamily and is highly expressed in cancer metastases.For better understanding of the role of PRL-3 in tumor metastasis,we applied a rapid and efficient method for generating PRL-3 floxed mice and investigated its phenotypes.A BAC retrieval strategy was applied to construct the PRL-3 conditional gene-targeting vector.Exon 4 was selected for deletion to generate a nonfunctional prematurely terminated short peptide as it will cause a frame-shift mutation.Conditional knockout PRL-3 mice were generated by using the Cre-loxP system and were validated by Southern blot and RT-PCR analysis.Further analysis revealed the phenotype characteristics of PRL-3 knockout mice and wildtype mice.In this study,we successfully constructed the PRL-3 conditional knockout mice,which will be helpful to clarify the roles of PRL-3 and the mechanisms in tumor metastasis.
机标关键词：PRL-3tumor metastasisprotein tyrosine phosphatasemiceretrieval strategyefficient methodanalysisunderstanding
分类号：R730.2(一般性问题)
资助基金：Grants from MOST of China(2005CB522501;2006BA123B00;;2006CB943503)
论文发表日期：2011-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息