Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)
Joshua Muli Mutiso
John Chege Macharia
Evans Taracha
Michael Muita Gicheru
摘要：In a previous immunogenicity and efficacy study in mice,montanide ISA 720 (MISA) was indicated to be a better adjuvant than bacillus calmette guerin vaccine (BCG) for a Leishmania vaccine.In the present study,we report the safety,immunogenicity and efficacy of Leishmania donovani (L.donovani) sonicated antigen delivered with alum-BCG (A1BCG),MISA or monophosphoryl lipid A (MPLA) in vervet monkeys following intradermal inoculums.Vaccinated and control animals were challenged with virulent L.donovani parasites and the parasitic burden was determined.Only animals vaccinated with alum-BCG adversely reacted to the inoculum by producing ulcerative erythematous skin indurations.Non-parametric ANOVA followed by a post test showed significantly higher IgG antibodies,and revealed the presence of lymphoproliferative and interferon gamma responses in both A1BCG+Ag and MISA+Ag as compared to the MPLA+Ag or other groups (P ＜ 0.001).We conclude that L.donovani sonicated antigen containing MISA is safe and is associated with protective immune response against Leishmania donovani infection in the vervet monkey model.
机标关键词：visceral leishmaniasisLeishmania donovanimonophosphoryl lipid Ainterferon gammaimmune responsestudyBCGcontrol
分类号：R392(医学免疫学)
资助基金：the National Council for Science and Technology,Government of Kenya(NCST 51003CALL2226)
论文发表日期：2012-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息