Expression, purification and characterization of Mycobacterium tuberculosis RpfE protein
Ying Xue
Yinlan Bai
Xue Gao
Hong Jiang
Limei Wang
Hui Gao
Zhikai Xu
摘要：Resuscitation promoting factor E (RpfE) is one of the five Rpf-like proteins in Mycobacterium tuberculosis (M.tuberculosis).These Rpf-like proteins are secretory,which make them candidates for recognition by the host immune system.In this study,the RpfE gene was amplified from M.tuberculosis,cloned into the expression vectors pDE22 and pPRO EXHT,and were expressed in Mycobacterium vaccae (M.vaccae) and Escherichia coli DH5α,respectively.Both recombinant RpfE proteins were purified by Ni-Sepharose affinity chromatography,and were given to C57BL/6 mice.The RpfE proteins elicited T cell proliferation,and stimulated the production of gamma interferon (IFN-γ),interleukin-10 (IL-10) and IL-12.Our results indicated that the RpfE protein expressed in M.vaccae could more efficiently stimulate cellular immune response,making it a promising candidate as a subunit vaccine.
机标关键词：Mycobacterium tuberculosiscellular immune responseaffinity chromatographyT cell proliferationEscherichia coliresultsC57BL/6making
分类号：R378(医学微生物学（病原细菌学、病原微生物学）)
资助基金：National Natural Science Foundation of China(30470097;30500432)
论文发表日期：2012-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息