Involvement of adenosine and standardization of aqueous extract of garlic (Allium sativum Linn.) on cardioprotective and cardiodepressant properties in ischemic preconditioning and myocardial ischemia-reperfusion induced cardiac injury
Ashish Kumar Sharma
Arshee Munajjam
Bhawna Vaishnav
Richa Sharma
Ashok Sharma
Kunal Kishore
Akash Sharma
Divya Sharma
Rita Kumari
Ashish Tiwari
Santosh Kumar Singh
Samir Gaur
Vijay Singh Jatav
Barthu Parthi Srinivasan
Shyam Sunder Agarwal
摘要：The present study investigated the effect of garlic (Allium sativum Linn.) aqueous extracts on ischemic preconditioning and ischemia-reperfusion induced cardiac injury,as well as adenosine involvement in ischemic preconditioning and garlic extract induced cardioprotection.A model of ischemia-reperfusion injury was established using Langendorff apparatus.Aqueous extract of garlic dose was standardized (0.5％,0.4％,0.3％,0.2％,0.1％,0.07％,0.05％,0.03％,0.01％),and the 0.05％ dose was found to be the most effective.Higher doses (more than 0.05％) were highly toxic,causing arrhythmia and cardiodepression,whereas the lower doses were ineffective.Garlic exaggerated the cardioprotective effect of ischemic preconditioning.The cardioprotective effect of ischemic preconditioning and garlic cardioprotection was significantly attenuated by theophylline (l,000 μmol/L) and 8-SPT (10 mg/kg,i.p.) and expressed by increased myocardial infarct size,increased LDH level,and reduced nitrite and adenosine levels.These findings suggest that adenosine is involved in the pharmacological and molecular mechanism of garlic induced cardioprotection and mediated by the modulation of nitric oxide.
机标关键词：aqueous extractischemic preconditioningmolecular mechanismLangendorffstudymodellevelfound
分类号：R541(心脏、血管（循环系）疾病)
论文发表日期：2012-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息