Parthenolide attenuates LPS-induced activation of NF-κB in a timedependent manner in rat myocardium
Hong Xie
Chen Wang
Xuemei Wu
Xia Liu
Shigang Qiao
Chunfeng Liu
Hong Liu
摘要：Parthenolide (PTN),a selective nuclear factor kappa B (NF-κB) inhibitor,has been used extensively to inhibit NF-κB activation.The duration of the inhibitory effect of PTN on NF-κB in vivo remains unclear.This study was to determine whether a lipopolysaccharide (LPS) challenge 6,12 and 24 h after the administration of PTN could activate NF-κB.Rats were devided into five groups.The rats in the PTN,PTN+LPS and DMSO groups were injected intraperitoneally with PTN or DMSO.After 6,12 or 24 h,LPS was administered in LPS and PTN+LPS groups.The expressions of NF-κB p50,IκBα and p-IκBα were inhibited in both PTN and PTN+LPS group at end of 6 and 12 h and no effects at 24 h.In summary,myocardial NF-κB expression occurs 1 h after the administration of LPS.PTN blocks this effect given at 6 h and no inhibitory effect 24 h after administration in vivo.
机标关键词：inhibitory effectPTNnuclear factor kappa BLPSchallengedurationDMSOsummary
分类号：R541(心脏、血管（循环系）疾病)
资助基金：National Natural Science Foundation of China(30872453)Natural Science Foundation of Jiangsu Province(BK2008166)Technology Bureau of Suzhou(2008-11)Natural Science Research Projects of Colleges and Universities in Jiangsu Province,China(08KJD320005)
论文发表日期：2012-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息