Soy isoflavone extracts stimulate the growth of nude mouse xenografts bearing estrogen-dependent human breast cancer cells (MCF-7)
Qian Wu
Ye Yang
Jing Yu
Nianzu Jin
摘要：We explored the effects of different lifetime exposures to soy isoflavone extracts on the growth of estrogendependent human breast cancer cells (MCF-7) implanted into athymic mice of different ovarian statuses.The athymic mice,ovariectomized or not,were implanted with MCF-7 cells.Mice were fed with low,moderate and high doses of soy isoflavone extract,at dietary concentrations of 6.25,12.5 and 25 g/kg,in different reproductive models,respectively.The expression of ki-67 was detected by immunohistochemistry.pS2 expression in tumors was analyzed by real-time PCR.Estrogen level in the serum was measured by chemiluminescence enzyme immunoassay.Total genistein and daidzein levels in serum and urine were determined by liquid chromatographyelectrospray tandem mass spectrometry (LC-ES/MS/MS).In Group A,on week 4,nude mice were exposed to different doses of soy iosflavone extracts.In Group B,the experimental diets were given to the nude mice following ovariectomy and rumor implantation.In both groups,6.25 and 12.5 g/kg soy isoflavone extracts stimulated the growth of MCF-7 xenografts,increased pS2 expression,proliferation and estrogen level in serum.In both Group B (postmenopausal mouse model) and Group C (premenopausal mouse model),soy isoflavone extracts at doses of 6.25 and 12.5 g/kg showed stimulatory effects on the growth of MCF-7 tumors.In conclusion,administration of soy isoflavone extracts at doses of 6.25 and 12.5 g/kg during adolescence or later in life stimulated tumor growth in both menopausal and postmenopausal mouse models.
机标关键词：breast cancer cellshumannude mousethe growthisoflavonedifferenttandem mass spectrometrymouse model
分类号：R737.9(泌尿生殖器肿瘤)
资助基金：National Natural Science Foundation of China(30572133)
论文发表日期：2012-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息