Cynodon dactylon and Sida acuta extracts impact on the function of the cardiovascular system in zebrafish embryos
Rajaretinam Rajesh Kannan
Samuel Gnana Prakash Vincent
摘要：The aim of the present study was to screen cardioactive herbs from Western Ghats of India.The heart beat rate (HBR) and blood flow during systole and diastole were tested in zebrafish embryos.We found that Cynodon dactylon (C.dactylon) induced increases in the HBR in zebrafish embryos with a HBR of (3.968±0.344) beats/s,which was significantly higher than that caused by betamethosone [(3.770±0.344) beats/s].The EC50 value of C.dactylon was 3.738 μg/mL.The methanolic extract of Sida acuta (S.acuta) led to decreases in the HBR in zebrafish embryos [(1.877±0.079) beats/s],which was greater than that caused by nebivolol (positive control).The EC50 value of Sida acuta was 1.195 μg/mL.The untreated embryos had a HBR of (2.685±0.160) beats/s at 3 d post fertilization (dpf).The velocities of blood flow during the cardiac cycle were (2,291.667 ± 72.169) μm/s for the control,(4,250± 125.000) μm/s for C.dactylon and (1,083.333±72.169) μm/s for S.acuta.The LC50 values were 32.6 μg/mL for C.dactylon and 20.9 μg/mL for S.acuta.In addition,the extracts exhibited no chemical genetic effects in the drug dosage range tested.In conclusion,we developed an assay that can measure changes in cardiac function in response to herbal small molecules and determine the cardiogenic effects by microvideography.
机标关键词：cardiovascular systemcardiac functionblood flowCynodon dactylongenetic effectscontrolduringdecreases
分类号：R93(生药学（天然药物学）)
论文发表日期：2012-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息