Resveratrol inhibits angiotensin Ⅱ-induced ERK1/2 activation by downregulating quinone reductase 2 in rat vascular smooth muscle cells
Xiwen Zhang
Yao Wang
Weiwei Yang
Xiaofeng Hou
Jiangang Zou
Kejiang Cao
摘要：Our previous studies showed that resveratrol could inhibit the proliferation of vascular smooth muscle cells (VSMCs) and repress mRNA and protein expression of quinone reductase 2 (NQO2).This study further explored the potential mechanisms whereby resveratrol inhibits the proliferation of rat VSMCs.Lentiviral vectors that incorporated NQO2 small interfering RNA (siRNA) were constructed and transduced into rat VSMCs.The cell proliferation was detected using the bromodeoxyuridine (BrdU) assay.Cultured rat VSMCs were stimulated with angiotensin Ⅱ and the level of reactive oxygen species (ROS) was measured using a ROS assay kit.A realtime quantitative PCR was used to detect NQO2 mRNA levels.Extracellular signal-regulated kinase (ERK1/2)and NQO2 protein expression were determined by Western blotting analysis.The inhibitory effect of resveratrol (10 and 50 μmol/L) on the proliferation of rat VSMCs in the NQO2 siRNA group was significantly weaker than that in the normal and scrambled siRNA group (P ＜ 0.01).The ROS level in the NQO2 siRNA and resveratrol (50 μmol/L) treatment groups were lower than that in the normal and scrambled siRNA groups (P ＜ 0.0l in both).Compared with the normal and scrambled siRNA group,the phosphorylation of ERK1/2 was significantly decreased in the NQO2 siRNA and resveratrol (50 μmol/L) treatment group (P ＜ 0.01 in both).In conclusion,high concentration of resveratrol inhibits angiotensin Ⅱ-induced ERK1/2 phosphorylation and subsequent proliferation by down-regulation of NQO2 in cultured rat VSMCs.
机标关键词：vascular smooth muscle cellsprotein expressionsiRNAVSMCsreactive oxygen speciessmall interfering RNAtreatmentERK1/2
分类号：R285(中药学)
论文发表日期：2012-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息