Effects of lysed Enterococcus faecalis FK-23 on experimental allergic rhinitis in a murine model
Luping Zhu
Takashi Shimada
Ruoxi Chen
Meiping Lu
Qingzhao Zhang
Wenmin Lu
Min Yin
Tadao Enomoto
Lei Cheng
摘要：In the current study,we sought to investigate whether lysed Enterococcus faecalis FK-23 (LFK),a heat-killed probiotic preparation,attenuated eosinophil influx into the upper airway and had immunomodulatory activity in a murine allergic rhinitis model.Eighteen BALB/c mice were divided into three groups; the ovalbumin (OVA)-sensitized/challenged group,which received saline orally for 6 weeks (OVA group),the OVA-sensitized/challenged group,which received LFK orally for 6 weeks (LFK-fed group),and the non-sensitized group,which received saline for 6 weeks (saline control group).Nasal rubbing and sneezing were monitored during the study.After the final challenge,interleukin (IL)-4,interferon (IFN)-γ,and OVA-specific IgE levels in the sera and splenocyte culture supernatants were determined,eosinophilic infiltrate into the upper airway was quantified,and splenic CD4+CD25+ regulatory T ceils (Tregs) were examined by flow cytometry.We found that nasal rubbing was significantly reduced in LFK-fed mice compared to the OVA group on d 27 and 35,and sneezing was significantly inhibited by LFK administration for 35 d.LFK-fed mice had significantly less eosinophil influx into the nasal mucosa than the OVA group.There were no significant differences between the LFK-fed group and OVA group in the serum and splenocyte culture supematant levels of IL-4,IFN-γ,and OVA-specific IgE.Interestingly,the LFK-fed mice had a significantly greater percentage of splenic CD4+CD25+ Tregs than OVA group.Our results indicate that oral administration of LFK may alleviate nasal symptoms,reduce nasal eosinophilia,and increase the percentage of CD4+CD25+ Tregs in experimental allergic rhinitis.
机标关键词：murine modelallergic rhinitisoral administrationflow cytometry
分类号：S858.28(动物医学（兽医学）)
资助基金：International Cooperation Program of Jiangsu Department of Science and Technology(BZ2011045)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD 2010-2013)the Health Promotion Project of Jiangsu Province(RC2007065;RC2011071)
论文发表日期：2012-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息