The role of aberrant promoter hypermethylation of DACT1 in bladder urothelial carcinoma
Huan Cheng
Zhonglei Deng
Zengjun Wang
Wei Zhang
Jiantang Su
1.Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China2.Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China3.Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China4.Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China5.Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China
摘要：The purpose of this study was to determine the relationship between hypermethylation of DACT1 gene promoter and lower mRNA expression in bladder urothelial carcinoma tissue.The methylation status of 29 urothelial carcinoma samples and 29 normal tissue samples were examined by methylation-specific polymerase chain reaction (MSP).The DACT1 mRNA transcript levels and DACT1 protein levels in all samples were then evaluated to define the relationship between the methylation status of the DACT1 promoter and its expression at the transcriptional and translational levels.Decreased expression of DACT1 was detected in 89.66％ of urothelial carcinomas (26/29; P ＜ 0.005).Promoter hypermethylation was found in 58.62％ (17/29) urothelial carcinomas and 25％ (7/29) normal tissues,respectively (P ＜ 0.05).DACT1 expression was lower in tissues where the DACT1 gene promoter was hypermethylated than in unmethylated tissues (0.25±0.17 vs 0.69±0.30,P ＜ 0.05).DACT1 gene hypermethylation was closely related to tumor size,grade and stage (P ＜ 0.05).Our results indicate that silencing and downregulation of DACT1 mRNA may be implicated in carcinogenesis and the progression of bladder urothelial carcinoma,and may be a potential prognostic factor.
机标关键词：urothelial carcinomapolymerase chain reactionprognostic factormRNA expressiontumor size
分类号：R737.14(泌尿生殖器肿瘤)
论文发表日期：2012-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息