Sox2 enhances the tumorigenicity and chemoresistance of cancer stem-like cells derived from gastric cancer
Tian Tian1
Yajie Zhang1
Shouyu Wang2
Jianwei Zhou2
Shan Xu1
1.Department of Cell Biology, Cancer Center, Nanjing Medical University, Nanjing, Jiangsu 210029, China2.Department of Molecular Cell Biology & Toxicology, School of Public Health, Nanjing Medical University, Nanjing,Jiangsu 210029, China
摘要：Gastric cancer stem-like cells (GCSCs) have been identified to possess the ability of self-renewal and tumor initiation.However,the mechanisms involved remain largely unknown.Here,we isolated and characterized the GCSCs by side population (SP) sorting procedure and cultured sphere cells (SC) from human gastric cancer cell lines SGC7901,BGC-823,MGC-803,HGC-27 and MKN-28.The sorting and culture assay revealed that SP cells proliferated in an asymmetric division manner.In addition,SP cells exhibited a higher potential of spheroid colony formation and greater drug resistance than non-SP cells (NSP).Moreover,the SC were found with enhanced capabilities of drug resistance in vitro and tumorigenicity in vivo.Sox2 mRNA and protein was highly and significantly overexpressed in the SP cells and SC.Importantly,downregulation of Sox2 with siRNA obviously reduced spheroid colony formation and doxorubicin effiux,as well as increased apoptosis rate in sphere cells in vitro and suppressed tumorigenicity in vivo.These results suggest that both SP cells and cultured SC enrich with GCSCs and that Sox2 plays a pivotal role in sustaining stem cell properties and might be a potential target for gastric cancer therapy.
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分类号：R735.2(消化系肿瘤)
论文发表日期：2012-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息