Overexpression of the mTERT gene by adenoviral vectors promotes the proliferation of neuronal stem cells in vitro and stimulates neurogenesis in the hippocampus of mice
Mengying Liu
Yao Hu
Lijuan Zhu
Chen Chen
Yu Zhang
Weixiang Sun
Qigang Zhou
1.Department of Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, Jiangsu 210029, China2.Department of Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, Jiangsu 210029, China3.Department of Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, Jiangsu 210029, China4.Department of Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, Jiangsu 210029, China5.Department of Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, Jiangsu 210029, China6.Department of Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, Jiangsu 210029, China7.Department of Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, Jiangsu 210029, China
摘要：We sought to construct the adenoviral vector carrying the gene encoding mouse telomerase reverse transcriptase (mTERT),as well as detect its expression and effect on the proliferation of neuronal stem cells.mTERT was amplified by RT-PCR and then the eukaryotic expression vector of pDC-EGFP-TERT was constructed.After DNA sequence analysis,we detected that there were 293 cells transfected with pDC-EGFP-TERT and helper adenovirus plasmid pBHG lox △EI,and three Cre using Lipofectamine 2000 mediation,named Ad-mTERT-GFP,to package adenoviral particles.The Ad-mTERT-GFP was used to infect neuronal stem cells and then the expression and activity of mTERT were detected.In addition,Bromodeoxyuridine labeling test identified the impact of mTERT overexpression on proliferation of neuronal stem cells.The recombinant adenoviral vector confirmed that mTERT was successfully constructed.Overexpression of mTERT stimulated the proliferation of neuronal stem cells both in vitro and in vivo.mTERT overexpression via adenoviral vector carrying mTERT cDNA upregulated the ability of proliferation in neuronal stem cells.
机标关键词：in vitrostem cellsadenoviral vectoreukaryotic expression vectorDNA sequence analysis
分类号：R329(人体形态学)
资助基金：the Science and Technology Foundation of Nanjing Medical University(09NJMUZ15)the Natural Science Foundation of Jiangsu Province(10KJB31008)
论文发表日期：2012-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息