MiRNA-429 suppresses the growth of gastric cancer cells in vitro
Di Liu1
Peng Xia1
Dongmei Diao1
Yao Cheng1
Hao Zhang1
Dawei Yuan1
Chen Huang2
Chengxue Dang1
1.Department of Surgical Oncology, the First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an,Shaanxi 710061, China2.Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University/Key Laboratory of Environment and Genes Related Diseases of Ministry of Education, Xi'an, Shaanxi 710061, China
摘要：Micro-RNAs (miRNAs) have been found to be implicated in a very wide range of physiological processes.This study was aimed to investigate the regulation of miRNA-429 (miR-429) in gastric cancer cells on cell proliferation and apoptosis.Quantitative PCR was employed to detect the expressions of miR-429 after eukaryotic expression plasmid of miR-429 and its inhibitor were transiently transfected into poorly differentiated human gastric cancer cell line BGC823.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assays were used to examine proliferation ability.Apoptosis was analyzed by flow cytometry after transfection.The results showed that 48 h after transfection,overexpression of miR-429 reached maximum efficiency.Compared with mock transfection,miR-429 inhibited tumor cell proliferation significantly (P ＜ 0.05) at 48 h and 72 h.of Overexpression of miR-429 promoted tumor cell apoptosis when compared with mock transfected cells (P ＜ 0.05).On the contrary,miR-429 inhibitor promoted tumor cell proliferation and inhibited apoptosis when compared with controls (P ＜ 0.05).Our results suggested that miRNA-429 may serve as a tumor suppressor during tumorigenesis of gastric cancer and may be a potential gastric cancer therapeutic target.
机标关键词：
分类号：R735.2(消化系肿瘤)
论文发表日期：2012-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息