Hydroxylation and sulfation of sex steroid hormones in inflammatory liver
Sang R.Lee
Seung-yeon Lee
Sang-yun Kim
Si-yun Ryu
Bae-kuen Park
Eui-Ju Hong
摘要：Sex steroids,also known as gonadal steroids,are oxidized with hydroxylation by cytochrome P450,glucuronidation by UDP-glucuronosyltransferase,sulfation by sulfotransferase,and O-methylation by catechol O-methyltransferase.Thus,it is important to determine the process by which inflammation influences metabolism of gonadal hormones.Therefore,we investigated the mechanism of metabolic enzymes against high physiologic inflammatory response in vivo to study their biochemical properties in liver diseases.In this study,C57BL/6N mice were induced with hepatic inflammation by diethylnitrosamine (DEN) exposure.We observed upregulation of Cyp 19a l,Hsd 17b 1,Cyp 1 a l,Sult1e 1 in the DEN-treated livers compared to the control-treated livers using real time PCR.Moreover,the increased Cyp 19al and Hsd 17b 1 levels support the possibility that estrogen biosynthesis from androgens are accumulated during inflammatory liver diseases.Furthermore,the increased levels of Cyplal and Cyplbl in the hydroxylation of estrogen facilitated the conversion of estrogen to 2-or 4-hydroxyestrogen,respectively.In addition,the substantial increase in the Sult1e1 enzyme levels could lead to sulfate conjugation of hydroxyestrogen.The present information supports the concept that inflammatory response can sequester sulfate conjugates from the endogenous steroid hormones and may suppress binding of sex steroid hormones to their receptors in the whole body.
机标关键词：inflammatory responseliver diseasesmetabolic enzymeswhole bodyreal time
分类号：R512.62(传染病)
论文发表日期：2017-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 437-444 )
英文信息