Effects of difructose dianhydride (DFA)-Ⅳ on in vitro fertilization in pigs
Young-Joo Yi
S.Kamala-Kannan
Jeong-Muk Lim
Byung-Taek Oh
Sang-Myeong Lee
摘要：Difructose dianhydride Ⅳ (DFA-Ⅳ) is produced from levan,which is a natural polysaccharide that belongs to the fructan family,through the activity of levan fructotransferase (LF) derived from microorganisms.Recently,DFA-Ⅳ has been expected to have diverse applications in the food and medical industry.Here,we examined the potential application of DFA-Ⅳ for in vitro fertilization (IVF) in pigs.In the assessment of acrosomal integrity during incubation,intact acrosomal or viable spermatozoa were highly sustained in 0.1％ or 0.25％ DFA-Ⅳ (69.8％-70.8％,P ＜ 0.05).Reactive oxygen species (ROS) levels during sperm incubation decreased following the addition of DFA-Ⅳ,and 0.1％-0.5％ DFA-Ⅳ in particular significantly decreased ROS production relative to that seen with no addition or 0.75％ DFA-Ⅳ.Total fertilization (mono + polyspermic oocyte) rate was significantly higher in the addition of 0.1％ DFA-Ⅳ (94.2％) than with other concentrations (71.8％-86.7％,P ＜ 0.05).When using reduced IVF times and lower sperm numbers,we found that addition of 0.1％-0.5％ DFA-Ⅳ significantly increased the fertilization rate (P＜ 0.05).Fertilized oocytes treated with 0.1％ DFA-Ⅳ exhibited higher embryonic development and blastocyst formation than those treated with other concentrations (P ＜ 0.05).Consequently,the addition of DFA-Ⅳ during IVF improved fertilization and embryonic development,suggesting the possible use of novel sugars for enhancement of assisted reproductive technology (ART) in mammals.
机标关键词：embryonic developmentin vitro fertilizationpotential applicationoxygen species
分类号：R715(临床优生学)
资助基金：We thank Hee-Jung Lee for assistance with the experiments,and Agency for Korea National Food Cluster for HPLC analysis.This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A6A3A-04063769)
论文发表日期：2017-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 453-461 )
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