Proteomic analysis of the dorsal spinal cord in the mouse model of spared nerve injury-induced neuropathic pain
Eun-sung Park
Jung-mo Ahn
Sang-min Jeon
Hee-jung Cho
Ki-myung Chung
Je-yoel Cho
Dong-ho Youn
摘要：Peripheral nerve injury often causes neuropathic pain and is associated with changes in the expression of numerous proteins in the dorsal horn of the spinal cord.To date,proteomic analysis method has been used to simultaneously analyze hundreds or thousands of proteins differentially expressed in the dorsal horn of the spinal cord in rats or dorsal root ganglion of rats with certain type of peripheral nerve injury.However,a proteomic study using a mouse model ofneuropathic pain could be attempted because of abundant protein database and the availability oftransgenic mice.In this study,whole proteins were extracted from the ipsilateral dorsal half of the 4th-6th lumbar spinal cord in a mouse model of spared nerve injury (SNI)-induced neuropathic pain.In-gel digests of the proteins size-separated on a polyacrylamide gel were subjected to reverse-phase liquid-chromatography coupled with electrospray ionization ion trap tandem mass spectrometry (MS/MS).After identifying proteins,the data were analyzed with subtractive proteomics using ProtAn,an in-house analytic program.Consequently,15 downregulated and 35 upregulated proteins were identified in SNI mice.The identified proteins may contribute to the maintenance ofneuropathic pain,and may provide new or valuable information in the discovery of new therapeutic targets for neuropathic pain.
机标关键词：neuropathic painmouse modelperipheral nerve injurylumbar spinal cordtandem mass spectrometryelectrospray ionizationdorsal horndorsal root ganglion
分类号：R741(神经病学)
资助基金：funded by the Ministry of Education(2015R1D1A1A01059432)
论文发表日期：2017-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 494-502 )
英文信息