Profiles of metabolic gene expression in the white adipose tissue, liver and hypothalamus in leptin knockout (Lep△I14/△I14) rats
Leijian Guan
Kaixuan Xu
Shuyang Xu
Ningning Li
Xinru Wang
Yankai Xia
Di Wu
摘要：Leptin deficiency is principally linked to metabolic disorders.Leptin knockout (Lep△I14/△I14) Sprague Dawley rats created by CRISPR/Cas9 is a new model to study metabolic disorders.We used a whole rat genome oligonucleotide microarray to obtain tissue-specific gene expression profiles of the white adipose tissue,liver and hypothalamus in Lep△I14△I14 and wild-type (WT) rats.We found 1,651 differentially expressed (enriched) genes in white adipose tissue,916 in the liver,and 306 in the hypothalamus in the Lep△I14/△I14 rats compared to WT.Gene ontology category and KEGG pathway analysis of the relationships among differentially expressed genes showed that these genes were represented in a variety of functional categories,including fatty acid metabolism,molecular transducers and cellular processes.The reliability of the data obtained from microarray was verified by quantitative real-time PCR on 14 representative genes.These data will contribute to a greater understanding of different metabolic disorders,such as obesity and diabetes.
机标关键词：adipose tissuedifferentially expressed genesoligonucleotide microarraygene expression profilespathway analysisfatty acidnew model
分类号：R965(药理学)
资助基金：We gratefully acknowledge the technical assistance of Ling Song.This work was supported by grants from the National Natural Science Foundation of China (81470150)
论文发表日期：2017-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：13( 528-540 )
英文信息