Tcf7l1 promotes transcription of Kruppel-like factor 4 during Xenopus embryogenesis
Qing Cao
Yan Shen
Wei Zheng
Hao Liu
Chen Liu
摘要：Kruppel-like factor 4 (Klf4) is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis. However, its regulation during embryogenesis is still unclear. Here, we report that Tcf711, a key downstream transducer of the Wnt signaling pathway, could promote Klf4 transcription and stimulate Klf4 promoter activity in early Xenopus embryos. Furthermore, cycloheximide treatment showed a direct effect on Klf4 transcription facilitated by Tcf711. Moreover, the dominant negative form of Tcf711 (dnTcf711),which lacks N-terminus of the β-catenin binding motif, could still activate Klf4 transcription, suggesting that this regulation is Wnt/β-catenin independent. Taken together, our results demonstrate that Tcf711 lies upstream of Klf4 to maintain its expression level during Xenopus embryogenesis.
机标关键词：
分类号：R34(基础医学)
资助基金：We thank Model Animal Research Center of Nanjing University for their assistance in data collection. This work was supported by the Start-up Funding of Henan University of Science and Technology (13480027) to Q. C. and the Key Science Foundation of Nanjing Medical University(2015NJMUZD002)the Natural Science Foundation of Higher Education Institutions of Jiangsu Province(16KJB-180020)Natural Science Foundation of Jiangsu Province(BK20171053)National Natural Science Funds of China (81702747)
论文发表日期：2018-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 215-221 )
英文信息