Prostate cancer tends to metastasize in the bone-mimicking microenvironment via activating NF-κB signaling
Haibo Tong
Chunlin Zou
Siyuan Qin
Jie Meng
Evan T.Keller
Jian Zhang
Yi Lu
摘要：Prostate cancer preferentially metastasizes to the bone.However,the underlying molecular mechanisms are still unclear.To explore the effects of a bone-mimicking microenvironment on PC3 prostate cancer cell growth and metastasis,we used osteoblast differentiation medium (ODM;minimal essential medium alpha supplemented with L-ascorbic acid) to mimic the bone microenvironment.PC3 cells grown in ODM underwent epithelial-mesenchymal transition and showed enhanced colony formation,migration,and invasion abilities compared to the cells grown in normal medium.PC3 cells grown in ODM showed enhanced metastasis when injected in mice.A screening of signaling pathways related to invasion and metastasis revealed that the NF-κB pathway was activated,which could be reversed by Bay 11-7082,a NF-κB pathway inhibitor.These results indicate that the cells in different culture conditions manifested significantly different biological behaviors and the NF-κB pathway is a potential therapeutic target for prostate cancer bone metastasis.
机标关键词：
分类号：R737.25(泌尿生殖器肿瘤)
资助基金：The authors thank Xia Liu and Xin Huang for discussion and editing.We would like to thank native speaker Katrien for English language editing.This work was supported by National Natural Science Foundation of China (NSFC)(81272415 and 81171993)NSFC Key Project(81130046)Guangxi Key Projects(2013-GXNSFEA053004)Guangxi Projects(2014GXNS-FDA118030)
论文发表日期：2018-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 343-353 )
英文信息