Fasudil alleviates LPS-induced lung injury by restoring aquaporin 5 expression and inhibiting inflammation in lungs
Jingjing Wang
Hui Kong
Jian Xu
Yanli Wang
Hong Wang
Weiping Xie
摘要：Fasudil,a selective rho kinase (ROCK) inhibitor,has been reported to play a beneficial role in systemic inflammation in acute lung injury,but its mechanism for ameliorating pulmonary edema and inflammation remains unclear.Using hematoxylin-and-eosin (H&E) staining,immunohistochemistry,enzyme-linked immunosorbent assay,quantitative real time PCR and Western blotting,we found that fasudil attenuated LPS-induced lung injury,decreased lung edema,and suppressed inflammatory responses including leukocyte infiltration and IL-6 production.Further,fasudil upregulated LPS-induced aquaporin 5 reduction and inhibited NF-κB activation in the lungs of mice.Our results suggest that fasudil could restore the expression of aquaporin 5 to eliminate LPS-induced lung edema and prevent LPS-induced pulmonary inflammation by blocking the inflammatory pathway.Collectively,blockade of the ROCK pathway by fasudil may be a potential strategy for the treatment of acute lung injury.
机标关键词：
分类号：R541.6(心脏、血管（循环系）疾病)
资助基金：This study was supported by the National Natural Science Foundation of China(81273571)Jiangsu Clinical Research Center for Respiratory Diseases Project under grant No.BL2012012,and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)(JX10231802)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 156-163 )
英文信息