Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression
Yuyu Xu
Pengqi Wang
Chaoqi Xu
Xiaoyun Shan
Qing Feng
摘要：Acrylamide,a potential carcinogen,exists in carbohydrate-rich foods cooked at a high temperature.It has been reported that acrylamide can cause DNA damage and cytotoxicity.The present study aimed to investigate the potential mechanism of human hepatocarcinoma HepG2 cell proliferation induced by acrylamide and to explore the antagonistic effects of a natural polyphenol curcumin against acrylamide via miR-21.The results indicated that acrylamide (≤100 μmol/L) significantly increased HepG2 cell proliferation and miR-21 expression.In addition,acrylamide reduced the PTEN expression in protein level,while induced the expressions of p-AKT,EGFR and cyclin D1.The PI3K/AKT inhibitor decreased p-AKT protein expression and inhibited the proliferation of HepG2 cells.In addition,curcumin effectively reduced acrylamide-induced HepG2 cell proliferation and induced apoptosis through the expression of miR-21.In conclusion,the results showed that acrylamide increased HepG2 cell proliferation via upregulating miR-21 expression,which may be a new target for the treatment and prevention of cancer.
机标关键词：
分类号：R817(放射医学)
资助基金：the National Natural Science Foundation of China(81472977)foundation from the Priority Academic Program Development of Jiangsu Higher Education Institutions,and Graduate Student Practice and Innovation Project of Jiangsu Province Ordinary University(SJZZ15_0117)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 181-191 )
英文信息