Effects of methotrexate on collagen-induced arthritis in male Wistar rats
Jin Seok Kang
摘要：To evaluate the effect of methotrexate on collagen-induced arthritis,micro-computed tomography (micro-CT) and histopathological analyses were used in male Wistar rats.Rats were divided randomly into three groups.Group 1 was treated with 0.9％ saline,and groups 2 and 3 were boosted with type Ⅱ collagen.From day 21 to 42,groups 1 and 2 were orally treated with 0.9％ saline and group 3 was orally treated with 1.5 mg/kg methotrexate.All rats were sacrificed at day 42 after the first collagen treatment.Micro-CT analyses showed bony parameters,such as bone volume and trabecular number,were decreased in group 2 compared to group 1,and these parameters were recovered in group 3.Histopathological examination and pathological parameter scoring showed that the knee joints of rats in group 2 had severe joint destruction,showing cartilage and bone erosion,enlarged cavities with inflammatory cell infiltration and activation of synovial fibroblasts.By contrast,these changes were reduced in group 3.Taken together,methotrexate treatment showed therapeutic potential in male rat collagen-induced arthritis model,and micro-CT analysis and histopathological tools could be integrated to assess the quantification/qualification of arthritic lesions.
机标关键词：
分类号：R593.22(全身性疾病)
资助基金：I would like to thank Ms.Young Hee Kim and Kyung A Kwak for their technical assistance.Funding for this paper was provided by Namseoul University (2018-100035)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 244-249 )
英文信息