Schizonepeta tenuifolia inhibits collagen stimulated platelet function via suppressing MAPK and Akt signaling
Bo-Ra Jeon
Muhammad Irfan
Minki Kim
Seung Eun Lee
Jeong Hoon Lee
Man Hee Rhee
摘要：The prevalence of cardiovascular diseases (CVDs) is increasing at a rapid pace in developed countries,and CVDs are the leading cause of morbidity and mortality.Natural products and ethnomedicine have been shown to reduce the risk of CVDs.Schizonepeta (S.) tenuifolia is a medicinal plant widely used in China,Korea,and Japan and is known to exhibit anti-inflammatory,antioxidant,and immunomodulatory activities.We hypothesized that given herbal plant exhibit pharmacological activities against CVDs,we specifically explored its effects on platelet function.Platelet aggregation was evaluated using standard light transmission aggregometry.Intracellular calcium mobilization was assessed using Fura-2/AM,and granule secretion (ATP release) was measured in a luminometer.Fibrinogen binding to integrin α Ⅱ b β3,was assessed using flow cytometry.Phosphorylation of mitogen-activated protein kinase (MAPK) signaling molecules and activation of the protein kinase B (Akt) was assessed using Western blot assays.S.tenuifolia,extract potently and significantly inhibited platelet aggregation,calcium mobilization,granule secretion,and fibrinogen binding to integrin α Ⅱ bβ3.Moreover,all extracts significantly inhibited MAPK and Akt phosphorylation.S.tenuifolia extract inhibited platelet aggregation and granule secretion,and attenuated collagen mediated GPVI downstream signaling,indicating the potential therapeutic effects of these plant extracts on the cardiovascular system and platelet function.We suggest that S.tenuifolia extract may be a potent candidate to treat platelet-related CVDs and to be used as an antiplatelet and antithrombotic agent.
机标关键词：
分类号：R541.4(心脏、血管（循环系）疾病)
资助基金：This research was supported by the National Research Foundation of Korea and grant funded by the Korean Government (MSIP)This research was supported by the National Research Foundation of Korea and grant funded by the Korean Government (2015R1D1-A1A09057204)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 250-257 )
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