Developmental changes in contraction of gastric smooth muscle cells in rats correlate with their differences in RhoA/ROCK pathway
Othman Al-Shboul
Mahmoud Alfaqih
Ayman Mustafa
Ahmed Al-Dwairi
Mohammad Alqudah
Saja Haifawi
Mohanad Alebbini
Mohammad Al-Zaareer
Khobaib Alahmar
摘要：Previous research has shown that smooth muscle of the stomach undergoes developmental changes in the intracellular regulatory mechanism responsible for the contractile process.Whether these developmental changes relate to differences in the expression and/or activity of the key enzymes regulating smooth muscle contraction has not been previously evaluated.Therefore,we aimed to examine the expression and activation of the small monomeric G protein "RhoA" and Rho kinase (ROCK) as well as their correlation with the contraction of gastric smooth muscle cells (GSMCs) in newborn vs.adult rats.Freshly isolated single GSMCs from Sprague-Dawley rats at 1 week (newborn) and 3 months (adult) of age were used in the study.Protein and mRNA expression levels of both ROCK2 and total RhoA were higher in adult compared to newborn rats.Moreover,acetylcholine (ACh)-induced contractions of GSMCs in adult rats were significantly higher than that in newborn animals.Meanwhile,ROCK and Rho activation was higher in adult stomach cells compared to newborn ones.Pretreatment of GSMCs with Y-27632,the ROCK inhibitor,significantly reduced ACh-induced contraction in both groups of cells and greatly abolished contractile differences.In conclusion,our results indicate that RhoA/ROCK pathway and contraction of stomach muscle cells are under developmental regulation.
机标关键词：
分类号：R333.2(人体生理学)
资助基金：Jordan University of Science & Technology,Irbid,Jordan (Grant 20180087)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 289-296 )
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