A novel long non-coding RNA NFIA-AS1 is down-regulated in gastric cancer and inhibits proliferation of gastric cancer cells
Jing Wang
Xuezhi He
Xiyi Lu
Muhammad Amin Karim
Dengshun Miao
Erbao Zhang
1.Departments of Anatomy, Histology and Embryology, the Research Center for Bone and Stem Cells, Nanjing Medical University, Nanjing, Jiangsu 211166, China2.Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210009, China3.Department of Epidemiology and Biostatistics, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment,Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical University, Nanjing, Jiangsu 211166, China
摘要：Gastric cancer is one of the most common malignant gastrointestinal tumors whose morbidity and mortality account for the second and third place respectively in malignant tumors in China.As an important participant in tumor biology,the abnormal expression of long non-coding RNA (lncRNAs) in cancer cells is closely related to the occurrence and development of tumors and plays the role of oncogenes or tumor suppressor genes.In this study,we identified a novel lncRNA NFIA antisense RNA 1 (NFIA-AS1) and explored its role and clinical significance in gastric cancer.Real-time quantitative PCR was performed to detect the expression of NFIA-AS 1 in tumor tissues and corresponding normal tissues from 42 pairs of gastric cancer samples.The lower expression of NFIA-AS1 was significantly associated with larger tumor size,lower histological grade,and advanced TNM stage.Kaplan-meier analysis showed that NFIA-AS1 expression could be used as an independent predictor of overall survival.We also demonstrated that overexpression of NFIA-AS1 significantly inhibited the proliferation of gastric cancer cells through affecting p 16 levels.In conclusion,our results suggest that the lncRNA NFIA-AS 1 may play the role of tumor suppressor gene,and serve as a biomarker for prognosis or progression of gastric cancer.
机标关键词：gastric cancer cellsgastrointestinal tumorstumor suppressor genesclinical significanceoverall survivalmalignant tumorstumor biologyantisense RNA
分类号：R735.2(消化系肿瘤)
资助基金：grants from the Postgraduate Research & Practice Innovation Program of Jiangsu Province(KYCX17_1301 to E.Z.)the National Natural Science Foundation of China(81730066 to D.M.)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 371-381 )
英文信息