Near-infrared fluorescent labeled CGRRAGGSC peptides for optical imaging of IL-11Rα in athymic mice bearing tumor xenografts
Tiannv Li
Jin Sun
Yao Hu
Min Yang
Haibin Shi
Lijun Tang
摘要：The interleukin-11 (IL-11) and the IL-11 receptor α-subunit (IL-11 Rα) have been demonstrated to regulate the invasion and proliferation of tumor cells.Our study intends to evaluate a noninvasive imaging of IL-11Rα expression in breast tumors using near-infrared (NIR) fluorescent dye Cy7-1abeled IL-11 mimic peptide CGRRAGGSC.This work evaluated the IL-11Rα expression of breast tumor cells and the binding status of this peptide to IL-11 Rα in vitro and in vivo by using Western blotting,immunofluorescence staining and near-infrared fluorescence imaging.Our biochemical study showed that IL-11Rα was overexpressed in breast tumor cells (MCF-7).The cell-binding assay demonstrated specific binding of peptide CGRRAGGSC to MCF-7 cells in vitro.In vivo imaging results showed that NIR fluorescent signals of Cy7-CGRRAGGSC were selectively accumulated in tumor and metabolic organs.While in the blocking experiment,free CGRRAGGSC obviously blocked the concentration of the Cy7-CGRRAGGSC in the tumors.These results suggested that IL-11Rα may be used as a potential target for noninvasive imaging in IL-11Rα overexpressed tumors.Furthermore,the imaging agent of near-infrared fluorescent dye Cy7-1abeled CGRRAGGSC is suitable for IL-11 Rα expression imaging study in vivo.
机标关键词：noninvasive imagingtumor cellsfluorescence imagingWestern blottingin vitromimic peptide
分类号：R730.43(一般性问题)
论文发表日期：2019-01-01
页数：7( 391-397 )
英文信息