Gelatin filter capture-based high-throughput sequencing analysis of microbial diversity in haze particulate matter
Meiqing Sun
Zhanlin Ding
Hong Wang
Guangping Yu
Zhe Feng
Bingzhi Li
Penghui Li
1.Wuqing District Center for Disease Control and Prevention, Tianjin 301700, China;Key Laboratory of Systems Bioengineering(Ministry of Education), School of Chemical Engineering and Technology,Tianjin University, Tianjin 300072, China2.Wuqing District Center for Disease Control and Prevention, Tianjin 301700, China3.Key Laboratory of Systems Bioengineering(Ministry of Education), School of Chemical Engineering and Technology,Tianjin University, Tianjin 300072, China4.School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China
摘要：Airborne particulate matter (PM),especially PM2.5,can be easily adsorbed by human respiratory system.Their roles in carrying pathogens for spreading epidemic diseases has attracted great concern.Herein,we developed a novel gelatin filter-based and culture-independent method for investigation of the microbial diversity in PM samples during a haze episode in Tianjin,China.This method involves particle capture by gelatin filters,filter dissolution for DNA extraction,and high-throughput sequencing for analysis of the microbial diversity.A total of 584 operational taxonomic units (OTUs) of bacteria and 370 OTUs of fungi at the genus level were identified during hazy days.The results showed that both bacterial and fungal diversities could be evaluated by this method.This study provides a convenient strategy for investigation of microbial biodiversity in haze,facilitating accurate evaluation of airborne epidemic diseases.
机标关键词：particulate mattermicrobial diversityrespiratory systemDNA extraction
分类号：R122.1(环境医学、环境卫生)
资助基金：Project of Science and Technology Development in Wuqing District,Tianjin(WQKJ201614)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 414-421 )
英文信息