Pleiotropic effects of apolipoprotein A-Ⅱ on high-density lipoprotein functionality, adipose tissue metabolic activity and plasma glucose homeostasis
Evangelia Zvintzou
Eva Xepapadaki
Christina Kalogeropoulou
Serafoula Filou
Kyriakos E.Kypreos
1.Department of Pharmacology, University of Patras Medical School, Rio Achaias, TK 26500, Greece2.Department of Pharmacology, University of Patras Medical School, Rio Achaias, TK 26500, Greece3.Department of Pharmacology, University of Patras Medical School, Rio Achaias, TK 26500, Greece4.Department of Pharmacology, University of Patras Medical School, Rio Achaias, TK 26500, Greece5.Department of Pharmacology, University of Patras Medical School, Rio Achaias, TK 26500, Greece
摘要：Apolipoprotein A-Ⅱ (APOA-Ⅱ) is the second most abundant apolipoprotein of high-density lipoprotein (HDL)synthesized mainly by the liver and to a much lesser extent by the intestine.Transgenic mice overexpressing human APOA-Ⅱ present abnormal lipoprotein composition and are prone to atherosclerosis,though in humans the role for APOA-Ⅱ in coronary heart disease remains controversial.Here,we investigated the effects of overexpressed APOA-Ⅱ on HDL structure and function,adipose tissue metabolic activity,glucose tolerance and insulin sensitivity.C57BL/6 mice were infected with an adenovirus expressing human APOA-Ⅱ or a control adenovirus AdGFP,and five days post-infection blood and tissue samples were isolated.APOA-Ⅱ expression resulted in distinct changes in HDL apoproteome that correlated with increased antioxidant and anti-inflammatory activities.No effects on cholesterol efflux from RAW 264.7 macrophages were observed.Molecular analyses in white adipose tissue (WAT) indicated a stimulation of oxidative phosphorylation coupled with respiration for ATP production in mice overexpressing APOA-Ⅱ.Finally,overexpressed APOA-Ⅱ improved glucose tolerance of mice but had no effect on the response to exogenously administered insulin.In summary,expression of APOAⅡ in C57BL/6 mice results in pleiotropic effects with respect to HDL functionality,adipose tissue metabolism and glucose utilization,many of which are beneficial to health.
机标关键词：
分类号：R363(病理学)
资助基金：We would like to thank Dr.Constantinos Tellis for advising us on the Lp-PLA2 activity assay and Ms.Odysseia Savvoulidou for technical assistance.This work was supported financially by the program "Support of Young Investigators" MIS ((No.5005458 that was co-financed by the Operational Program "Human)
论文发表日期：2020-01-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：13( 14-26 )
英文信息