GSH-responsive curcumin/doxorubicin encapsulated Bactrian camel serum albumin nanocomposites with synergistic effect against lung cancer cells
Xinyu Yu1
Adilijiang Xieripu1
Qilan Xu1
Azhati Zulipikaer2
Yiyan Song1
Ling Cai1
Jin Chen3
1.School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China2.Xinjiang Academy of Animal Science, Urumqi, Xinjiang 830011, China3.School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China;The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China;Center for Global Health,School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China;National Laboratory of Biomacromolecules, Institute of Biop
摘要：The aim of this study was to prepare camel serum albumin (CSA) nanoparticles using a self-assembly strategy to co-immobilize curcumin (CCM) and doxorubicin (Dox) which was in favor of combined chemotherapy and biomedical applications of bactrian (Camelus bactrianus) CSA.The constructed CSA nanoparticles (CSA-NPs) with the size around 200 nm displayed a high degree ofpolydispersity and further encapsulation of CCM and Dox caused no apparent morphological changes to the nanocomposite (CCM/Dox CSA-NPs).The synergistic cytotoxic effect of CCM and Dox on cancer cell A549 was observed with the calculated combination index less than 1.0.Moreover,the release kinetic profile of encapsulated drugs showed a concentration dependence of glutathione (GSH) originating from the GSH used in nanoparticle formation to break the intramolecular disulfide bonds.In vitro cytotoxicity evaluations also revealed that CCM/Dox CSA-NPs showed higher cytotoxicity than that of single drug loaded CSA-NPs,which was also validated by high content screen assay.Taken together,the CCM/Dox CSA-NPs with redox-responsive attributes provided an integrated protein-based combinational drugdelivery matrix to exert synergistic effects.
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分类号：R734.2(呼吸系肿瘤)
论文发表日期：2020-01-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：13( 54-66 )
英文信息