Microarray expression profile and functional analysis of circular RNAs in choroidal neovascularization
Jiancheng Huang1
Meng Chen2
Kai Xu2
Rongmei Zhou2
Shujie Zhang2
Chen Zhao3
1.Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical University, State Key Laboratory of Reproductive Medicine, Nanjing, Jiangsu 210029, China;Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China2.Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China;NHC Key Laboratory of Myopia(Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, and Shanghai Key Laboratory of Visual Impairment and Restoration(Fudan University), Shanghai 200032,China3.Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical University, State Key Laboratory of Reproductive Medicine, Nanjing, Jiangsu 210029, China;Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China;NHC Key Laboratory of Myopia(Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, and Shanghai Key
摘要：Choroidal neovascularization (CNV) is a leading cause of visual loss in age-related macular degeneration (AMD).However,the molecular mechanism for CNV progression is still unclear.This study aimed to identify CNV-related circular RNAs (circRNAs),a novel class of non-coding RNAs with diverse functions.A total of 117 circRNAs were differentially expressed in the murine CNV model by microarrays.Gene ontology (GO)enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed to identify the functions of selected circRNAs.The host genes of these circRNAs were predicted to be targeted to neurogenesis (ontology:biological process),proteinaceous extracellular matrix (ECM) (ontology:cellular component),and binding (ontology:molecular function).Differentially expressed circRNAs-mediated regulatory networks were enriched in ECM receptor interaction.Most of the dysregulated circRNAs could potentially bind to five different miRNAs by TargetScan and miRanda.Specifically,circ_15752 was identified in this circRNAs pool which may facilitate vascular endothelial cell proliferation,migration,and tube formation,suggesting a critical role in endothelial angiogenesis.Our work suggests that dysregulated circRNAs may be involved in CNV pathogenesis and serve as potential biomarkers for CNV.
机标关键词：
分类号：R774.1(视网膜及视神经疾病)
论文发表日期：2020-01-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 67-74 )
英文信息