Pre-evaluation of humoral immune response of Bactrian camels by the quantification of Th2 cytokines using real-time PCR
Xinyu Yu1
Yuan Wu2
Jiarong Zhang1
Jirimutu3
Azhati Zulipikaer4
Jin Chen5
1.Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China2.Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, the Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, Jiangsu 210009, China3.Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China4.Xinjiang Academy of Animal Science, Urumqi, Xinjiang 830011, China5.Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China;The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China;Key Laboratory of Antibody Technique of National Health Commission, Nanjing Medical University, Nanjing, Jiangsu 211166, China
摘要：With the increasing immunological studies on camels due to the advantage of their single-chain antibodies for humanizations,it is demanding to develop an easy-to-handle evaluation method of their humoral immune response before proceeding with immunization of foreign antigens that may be toxic to camels.In this study,we quantitatively determined the expression levels of T-helper 2 (Th2) cytokines in peripheral blood lymphocytes obtained from Bactrian camels by real-time PCR.The recorded kinetic profiles resulting from the immunization of ovalbumin (OVA) indicated that after immunization,Th2 cytokines including interleukin (IL) families such as IL-4,IL-10,and IL-13 in the camels were up-regulated by a factor of 1.78,3.15,and 1.22,respectively,which was validated by traditional enzyme-linked immunosorbent assay (ELISA) methods.Unlike ELISA which requires specific enzyme-labeled antibodies,this established method based on the minimal amount of blood samples holds an advantage in the preliminary evaluation of camel humoral immune response with desirable precision,which is meaningful for biomedical explorations of camel-derived antibodies.
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分类号：R392.1(医学免疫学)
论文发表日期：2020-09-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 387-394 )
英文信息