The testis-specifically expressed gene Trim69 is not essential for fertility in mice
Xi He
Wenxiu Xie
Huiling Li
Yiqiang Cui
Ya Wang
Xuejiang Guo
Jiahao Sha
1.State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Naning, Jiangsu 211166, China2.State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Naning, Jiangsu 211166, China3.State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Naning, Jiangsu 211166, China4.State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Naning, Jiangsu 211166, China5.State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Naning, Jiangsu 211166, China6.State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Naning, Jiangsu 211166, China7.State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Naning, Jiangsu 211166, China
摘要：Protein ubiquitination is essential for diverse cellular functions including spermatogenesis.The tripartite motif(TRIM) family proteins,most of which have E3 ubiquitin ligase activity,are highly conserved in mammals.They are involved in important cellular processes such as embryonic development,immunity,and fertility.Our previous studies indicated that Trim69,a testis-specific expressed TRIM family gene,potentially participates in the spermatogenesis by mediating testicular cells apoptosis.In this study,we investigated the biological functions of Trim69 in male mice by established Trim69 knockout mice with CRISPR/Cas9 genomic editing technology.Here,we reported that the male Trim69 knockout mice had normal fertility.The adult knockout mice have shown that the appearance of testes,testis/body weight ratios,testicular histomorphology,and the number and quality of sperm were consistent with wild-type mice.These results indicated that the E3 ubiquitin ligase protein Trim69 was not essential for male mouse fertility,and it might be compensated by other TRIM family members such as Trim58 in Trim69-deficiency testis.This study would help to elucidate the functions of tripartite motif protein family and the regulation of spermatogenesis.
机标关键词：
分类号：R321.1(人体形态学)
资助基金：This work was supported by the National Key Research and Development Program of China (No.2018YFC1003503-2.No.2017YFA0103803.and No.2016YFA0500903)and the National Natural Science Foundation of China (No.31890784)
论文发表日期：2021-01-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：14( 47-60 )
英文信息