Designing and generating a mouse model: frequently asked questions
Channabasavaiah B.Gurumurthy1
Thomas L.Saunders2
Masato Ohtsuka3
1.Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68106-5915, USA;Mouse Genome Engineering Core Facility, Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68106-5915, USA2.Department of Internal Medicine, Division of Genetic Medicine, University of Michigan Medical School, Ann Arbor, MI48109, USA;Transgenic Animal Model Core, University of Michigan, Ann Arbor, M148109, USA3.Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan;The Institute of Medical Sciences, Tokai University, Isehara, Kanagawa 259-1193, Japan
摘要：Genetically engineered mouse (GEM) models are commonly used in biomedical research.Generating GEMs involve complex set of experimental procedures requiring sophisticated equipment and highly skilled technical staff.Because of these reasons,most research institutes set up centralized core facilities where custom GEMs are created for research groups.Researchers,on the other hand,when they begin thinking about generating GEMs for their research,several questions arise in their minds.For example,what type of model(s) would be best useful for my research,how do I design them,what are the latest technologies and tools available for developing my model(s),and finally how to breed GEMs in my research.As there are several considerations and options in mouse designs,and as it is an expensive and time-consuming endeavor,careful planning upfront can ensure the highest chance of success.In this article,we provide brief answers to several frequently asked questions that arise when researchers begin thinking about generating mouse model(s) for their work.
机标关键词：
分类号：Q789(基因工程（遗传工程）)
论文发表日期：2021-03-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：15( 76-90 )
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