Harnessing CRISPR-Cas system diversity for gene editing technologies
Alexander McKay
Gaetan Burgio
Department of Immunology and Infectious Diseases, John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia
摘要：The discovery and utilization of RNA-guided surveillance complexes,such as CRISPR-Cas9,for sequence-specific DNA or RNA cleavage,has revolutionised the process of gene modification or knockdown.To optimise the use of this technology,an exploratory race has ensued to discover or develop new RNA-guided endonucleases with the most flexible sequence targeting requirements,coupled with high cleavage efficacy and specificity.Here we review the constraints of existing gene editing and assess the merits of exploiting the diversity of CRISPR-Cas effectors as a methodology for surmounting these limitations.
机标关键词：
分类号：Q789(基因工程（遗传工程）)
论文发表日期：2021-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：16( 91-106 )
英文信息