A transgenic pig model expressing a CMV-ZsGreen1 reporter across an extensive array of tissues
Amy T.Desaulniers1
Rebecca A.Cederberg1
Elizabeth P.Carreiro1
Channabasavaiah B.Gurumurthy2
Brett R.White1
1.Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583-0908, USA2.Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198-5930, USA
摘要：Since genetic engineering of pigs can benefit both biomedicine and agriculture,selecting a suitable gene promoter is critically important.The cytomegalovirus (CMV) promoter,which can robustly drive ubiquitous transgene expression,is commonly used at present,yet recent reports suggest tissue-specific activity in the pig.The objective of this study was to quantify ZsGreen1 protein (in lieu of CMV promoter activity) in tissues from pigs harboring a CMV-ZsGreen1 transgene with a single integration site.Tissue samples (n=35) were collected from neonatal hemizygous (n=3) and homozygous (n=3) piglets and ZsGreen1 abundance was determined via immunoblotting.ZsGreenl was detected in all tissues,except hypothalamus,kidney cortex and oviduct.The expression patterns of homozygous and hemizygous piglets were similar (P＞0.05).However,quantification revealed that ZsGreen1 protein levels were tissue-specific.Within neural/endocrine tissues,ZsGreenl abundance was highest in the anterior pituitary gland,intermediate in the cerebellum and lowest in the cerebrum,spinal cord and posterior pituitary (P＜0.05).In the digestive system,ZsGreenl was more abundant in the salivary gland than esophagus,stomach,pancreas,duodenum,jejunum,ileum,spleen,colon,gallbladder and liver (P＜0.05).Interestingly,ZsGreenl amounts also differed within an organ (i.e.,the right ventricle had 3-fold higher levels than the other heart chambers;P＜0.05).These results provide useful information for the use of the CMV promoter to drive transgene expression in the pig.Moreover,this swine model represents a novel resource of ZsGreenllabeled organs and a valuable tool to advance genome editing research.
机标关键词：
分类号：Q789(基因工程（遗传工程）)
论文发表日期：2021-03-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 163-173 )
英文信息