Triclosan inhibits the activation of human periodontal ligament fibroblasts induced by lipopolysaccharide from Porphyromonas gingivalis
Wei Shu1
Yanman Zhang2
Chen Zhang3
Qiang You3
Hong Zhou4
Shuang Wen2
1.Department of Immunology,School of Basic Medical Sciences,Nanjing Medical University,Nanjing,Jiangsu 211166,China;Department of Stomatology,Jiangsu Provincial Hospital of Traditional Chinese Medicine,Nanjing,Jiangsu 210029,China2.Department of Immunology,School of Basic Medical Sciences,Nanjing Medical University,Nanjing,Jiangsu 211166,China3.Department of Biotherapeutics,the Second Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210011,China4.Department of Immunology,School of Basic Medical Sciences,Nanjing Medical University,Nanjing,Jiangsu 211166,China;Department of Biotherapeutics,the Second Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210011,China
摘要：Periodontitis is a highly prevalent,chronic,non-specific,and immunologically devastating disease of periodontal tissues,caused by microbial infection.This study aims to examine the efficacy and protective mechanism of triclosan(TCS),a bisphenolic,non-cationic component of oral care products,against periodontal inflammation induced by lipopolysaccharide purified from Porphyromonas gingivalis(LPS-PG).TCS markedly downregulated interleukin-6(IL-6),IL-8,and IL-15 in human periodontal ligament fibroblasts(HPDLFs)treated with LPS-PG.By using a liquid chromatography-tandem mass spectrometry(LC-MS/MS)approach,318 differentially expressed proteins(161 upregulated and 157 downregulated)were identified in TCS-pretreated HPDLFs.TCS upregulated HSPA5 and HSP90B1 but downregulated HSPA2.Besides,TCS upregulated miR-548i in HPDLFs,which downregulated IL-15.These results indicate that TCS attenuates the activation of HPDLFs and downregulates the inflammatory cytokines through various mechanisms,thus highlighting its protective role in periodontal inflammation.
机标关键词：
分类号：R781.4(口腔内科学)
论文发表日期：2021-05-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 206-215 )
英文信息