SARS-CoV-2 encoded microRNAs are involved in the process of virus infection and host immune response
Zhi Liu1
Jianwei Wang1
Yiyue Ge2
Yuyu Xu1
Mengchen Guo1
Kai Mi1
Rui Xu1
Yang Pei1
Qiankun Zhang1
Xiaoting Luan1
Zhibin Hu3
Ying Chi2
Xingyin Liu4
1.State Key Laboratory of Reproductive Medicine,Center of Global Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China;Department of Pathogen Biology-Microbiology Division,Key Laboratory of Pathogen of Jiangsu Province,School of Basic Medical Science,Nanjing Medical University,Nanjing,Jiangsu 211166,China2.National Health Commission Key Laboratory of Enteric Pathogenic Microbiology,Jiangsu Provincial Center for Diseases Control and Prevention,Nanjing,Jiangsu 210009,China3.State Key Laboratory of Reproductive Medicine,Center of Global Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China4.State Key Laboratory of Reproductive Medicine,Center of Global Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China;Department of Pathogen Biology-Microbiology Division,Key Laboratory of Pathogen of Jiangsu Province,School of Basic Medical Science,Nanjing Medical University,Nanjing,Jiangsu 211166,China;Key Laboratory of Human Functional Genomics of Jiangsu Province,Nanjing Medical University,Nanjing,Jiangsu 211166,China
摘要：The outbreak of COVID-19 caused by SARS-CoV-2 is spreading worldwide,with the pathogenesis mostly unclear.Both virus and host-derived microRNA(miRNA)play essential roles in the pathology of virus infection.This study aims to uncover the mechanism for SARS-CoV-2 pathogenicity from the perspective of miRNA.We scanned the SARS-CoV-2 genome for putative miRNA genes and miRNA targets and conducted in vivo experiments to validate the virus-encoded miRNAs and their regulatory role on the putative targets.One of such virus-encoded miRNAs,MR147-3p,was overexpressed that resulted in significantly decreased transcript levels of all of the predicted targets in human,i.e.,EXOC7,RAD9A,and TFE3 in the virus-infected cells.The analysis showed that the immune response and cytoskeleton organization are two of the most notable biological processes regulated by the infection-modulated miRNAs.Additionally,the genomic mutation of SARS-CoV-2 contributed to the changed miRNA repository and targets,suggesting a possible role of miRNAs in the attenuated phenotype of SARS-CoV-2 during its evolution.This study provided a comprehensive view of the miRNA-involved regulatory system during SARS-CoV-2 infection,indicating possible antiviral therapeutics against SARS-CoV-2 through intervening miRNA regulation.
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分类号：R511(传染病)
论文发表日期：2021-05-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：12( 216-227 )
英文信息