Construction of miRNA-mRNA network reveals crucial miRNAs and genes in acute myocardial infarction
Kai Wang1
Zhongming Li2
Wenjie Ma2
Yan Sun2
Xianling Liu2
Lijun Qian2
Jian Hong2
Dasheng Lu3
Jing Zhang1
Di Xu2
1.Department of Cardiology,the First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China2.Department of Geriatrics,the First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China3.Department of Cardiology,the Second Affiliated Hospital of Wannan Medical College,Wuhu,Anhui 241000,China
摘要：Acute myocardial infarction (AMI) is a severe cardiovascular disease.This study aimed to identify crucial microRNAs (miRNAs) and mRNAs in AMI by establishing a miRNA-mRNA network.The microarray datasets GSE31568,GSE148153,and GSE66360 were downloaded from the Gene Expression Omnibus (GEO) database.We identified differentially expressed miRNAs (DE-miRNAs) and mRNAs (DE-mRNAs) in AMI samples compared with normal control samples.The consistently changing miRNAs in both GSE31568 and GSE148153 datasets were selected as candidate DE-miRNAs.The interactions between the candidate DE-miRNAs and DE-mRNAs were analyzed,and a miRNA-mRNA network and a protein-protein interaction network were constructed,along with functional enrichment and pathway analyses.A total of 209 DE-miRNAs in the GSE31568 dataset,857 DE-miRNAs in the GSE148153 dataset,and 351 DE-mRNAs in the GSE66360 dataset were identified.Eighteen candidate DE-miRNAs were selected from both the GSE31568 and GSE148153 datasets.Furthermore,miR-646,miR-127-5p,miR-509-5p,miR-509-3-5p,and miR-767-5p were shown to have a higher degree in the miRNA-mRNA network.THBS-1 as well as FOS was a hub gene in the miRNA-mRNA network and the protein-protein interaction (PPI) network,respectively.CDKN1A was important in both miRNA-mRNA network and PPI network.We established a miRNA-mRNA network in AMI and identified five miRNAs and three genes,which might be used as biomarkers and potential therapeutic targets for patients with AMI.
机标关键词：
分类号：R542.2(心脏、血管（循环系）疾病)
论文发表日期：2021-11-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 425-435 )
英文信息