Identification of biomarkers of male infertility through the circRNA expression profiling of seminal plasma
Zhaode Liu1
Xinrui Li1
Xiaoyu Yang2
Bohang Zhang1
Dingdong Chen1
Yan Yuan1
Yiqiang Cui1
1.State Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China2.State Key Laboratory of Reproductive Medicine and Offspring Health,Clinical Center of Reproductive Medicine,the First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China
摘要：Circular RNAs(circRNAs)are key regulators of reproductive biology.However,limited information is available regarding circRNA expression profiles in seminal plasma samples from individuals with male infertility.The present study aimed to identify circRNAs associated with infertility in seminal plasma samples and to clarify their potential as biomarkers,as well as the possible molecular mechanisms underlying their functions.Next-generation RNA sequencing was conducted to analyze circRNA profiles in seminal plasma from healthy controls,oligoasthenospermia(OAZ)patients,and non-obstructive azoospermia(NOA)patients.Bioinformatics analysis revealed that 637 circRNAs were differentially expressed between OAZ and control subjects,and 272 circRNAs were differentially expressed between NOA and control subjects.The expression of key circRNAs(hsa-SAP130_0002,hsa-TRPC1_0001,hsa-FBRS_0001,hsa-ACACA_0025,hsa-UTRN_0042,and hsa-ZNF532_0023)was then validated by qPCR,and their diagnostic accuracy for infertility was confirmed through receiver operating characteristic curve analysis.Additionally,a possible circRNA-miRNA-mRNA regulatory network was constructed for these candidate biomarkers.Collectively,this study identifies a novel set of circRNAs with potential as diagnostic biomarkers for male infertility and provides molecular insights that may facilitate both diagnostic and therapeutic efforts.
机标关键词：identificationexpressionprofilingcircrnathroughplasmabiomarkersinfertility
分类号：R698.2(泌尿科学（泌尿生殖系疾病）)
论文发表日期：2025-07-30
在线出版日期：2025-10-11（本平台首次上网日期，不代表文献的发表时间）
页数：15( 367-381 )
英文信息