DOI: 10.1515/mr-2024-0039
A stepwise approach to deriving functional β-cells from human embryonic or induced pluripotent stem cells
Clara Farhat1
Viktoria Xega1
Jun-Li Liu2
1.The Research Institute of McGill University Health Centre,Montreal,Canada;Department of Medicine,Division of Endocrinology and Metabolism,McGill University,Montreal,Canada2.The Research Institute of McGill University Health Centre,Montreal,H3A 0G4,Canada;Department of Medicine,Division of Endocrinology and Metabolism,McGill University,Montreal,H3A0G4,Canada
摘要：Our understanding of β-cell differentiation from pluripotent stem cells(PSCs)is rapidly evolving.Although progress has been made,challenges remain,particularly in achieving glucose-stimulated insulin secretion(GSIS).Human embryonic stem cells(hESCs)are valuable due to their plurip-otent ability.A fixed protocol targeting master regulatory genes initiates stem cells into pancreatic lineage commitment.Due to the observations that a single stem cell can differentiate into multiple cell types depending on various factors and conditions,non-linear differentiation pathways exist.Co-expression of key factors remains essential for successful β-cell differentiation.The mature β-cell marker MAFA plays a critical role in maintaining the differentiation state and preventing dedif-ferentiation.Recapitulating pancreatic islet clustering en-hances physiological responses,offering potential avenues for diabetes treatment.On the other hand,several enhanced differentiation protocols from induced pluripotent stem cells(iPSCs)have improved the functional insulin produc-ing β-cells generated.These findings,with their potential to revolutionize diabetes treatment,highlight the complexity of β-cell differentiation and guide further advancements in regenerative medicine.
机标关键词：pluripotentcellsstemhumanfromapproachderivingembryonic
论文发表日期：2025-02-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：12( 23-34 )
英文信息