大鼠颅骨骨缺损模型的建立及相关应用
李矛1
赵彦涛2
侯树勋2
1.100048 北京,解放军总医院第一附属医院、北京市骨科植入医疗器械工程技术研究中心、全军骨科研究所;100048 北京,解放军第 306 医院2.解放军总医院第一附属医院、北京市骨科植入医疗器械工程技术研究中心、全军骨科研究所, 北京,100048
摘要：The rat calvarial bone defect model is widely applied in bone tissue engineering, especially in the animal model for bone grafting materials and relative cytokines. There are many disputes on the diameter size of the critical size defects ( CSD ), unilateral or bilateral defects, whether to shield the dura mater and pericranium during the process of establishing the rat calvarial bone defect model. Based on our literature review, there are many different disputes about the species, sex, and age of rats. We've confirmed that male adult ( 10 - 12 weeks, 300 - 350g , Sprague Dawley / SD ) rats are preferred in the model establishment. When the CSD is 5 mm, the researcher can make a self-comparison and avoid the damage of the sagittal suture, thus reduce the number of rats with the bilateral defects at the same time. When the CSD is 8 mm, a larger range of defects may be established, so as to make the experimental results more convincing. The dura mater and pericranium can promote the repair of calvarial bone defects as intramembranous bone growth sites. Therefore, we may consider shielding the dura mater and pericranium during the process of evaluating the bone repair materials and relative cytokines to avoid the interference on the results.
关键词：大鼠颅骨骨缺损组织工程
分类号：R683(骨科学（运动系疾病、矫形外科学）)R318(医用一般科学)
论文发表日期：2017-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 629-633 )
英文信息