微创开颅法局灶性大鼠脑梗死模型的实验研究
薛静
高培毅
安沂华
SUN Chong-ran
LI Jin
HUANG Hua
1.The Department of Neuroradiology, the Affiliated Beijing Tiantan Hospital of Capital University of Medical Sciences, Beijing 100050, China2.The Department of Neuroradiology, the Affiliated Beijing Tiantan Hospital of Capital University of Medical Sciences, Beijing 100050, China3.The Department of Neuroradiology, the Affiliated Beijing Tiantan Hospital of Capital University of Medical Sciences, Beijing 100050, China4.The Department of Neuroradiology, the Affiliated Beijing Tiantan Hospital of Capital University of Medical Sciences, Beijing 100050, China5.The Department of Neuroradiology, the Affiliated Beijing Tiantan Hospital of Capital University of Medical Sciences, Beijing 100050, China6.The Department of Neuroradiology, the Affiliated Beijing Tiantan Hospital of Capital University of Medical Sciences, Beijing 100050, China
摘要：目的建立一种稳定的大鼠脑局灶性梗死模型,以用于脑梗死后神经干细胞移植的长期观察.方法 37只大鼠随机分为实验组和对照组.微创法经颞骨局部钻孔开颅,采用直接结扎大脑中动脉终段,同时永久结扎同侧颈总动脉、暂时性夹闭对侧颈总动脉的方法制备大鼠脑梗死模型.通过大鼠脑梗死后的神经功能评分、墨汁灌注、TTC染色、MRI成像结果对该模型进行评价.结果大鼠术后状态良好,实验组大鼠观察4周后死亡率低为6.25%.大鼠神经功能评分均为1分,墨汁灌注及TTC染色观察梗死范围局限于皮层,4周后MRI成像测量梗死体积稳定,平均为83.52mm3.结论该模型对大鼠创伤小,梗死灶的位置和体积恒定,长期存活率高,为脑梗死后神经干细胞移植的研究提供了一种理想的动物模型.
关键词：脑梗死动物模型大脑中动脉大鼠
分类号：R743.3(神经病学与精神病学)
资助基金：北京市自然科学基金(7062031)中国科学院项目（非规范项目）(3037042)
论文发表日期：2006-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 11-13 )
英文信息