Effect of epidermal growth factor on the migration of neural stem cells

Faliang Duan; Guoping Yang; Junwu Wei; Jinglei Wu

摘要：BACKGROUND:Recently,researches on neural stem cells(NSCs)are focus on differentiation and migration of stem cells.How to regulate and control differentiation and migration of NSCs based on human wills is still a hot topic.OBJECTIVE:To investigate the effct of epidermal growth factor (EGF) on the migration and proliferation of NSCs and analyze duration of the effect.DESIGN:Contrast study based on cells.SETFING:Department of Neurological Surgery,the First Hospital of Wuhan.MATERIALS:Healthy SD rats aged 13-14 embryonic days.EGF(Sigma Company).METHODS:The experiment was carried out in the Animal Laboratory of Experimental Center Affiliated to the First Hospital of Wuhan from October 2004 to July 2006.NSCs selected from embryonic striatum of rats with 13-14 embryonic days were cultured;7 days later,suspended neural sphere was used to make simple cell suspension and cultured once more.Then,DMEM-F12+20 μg/L EGF was added into culture medium;14 days latar.the rats were divided into experimental group and control group.Rats in the experimental group were cultured with the same medium mentioned above;however, rats in the control group were cultured with only DMEM-F12.Migration of cells was observed under microscope every day.MAIN OUTCOME MEASURES:NSCs migration in both experimental group and control group.RESULTS:Cell spheres in primary culture were NSCs.In addition,14 days later,proliferation of stem cells were observed remarkably in EGF culture.and size of cell sphere was about that of 100 cells.In exparimental group.proliferation of cell sphere was slow down on the 14th culture day,and apophysis was erupted to neighbor cell sphere.Moreover,NSCs migrated from big cell sphere to small cell sphere during 14-17 culture days.and then,cell migration was disappeared at 17 days after culture.In control group.cell migration was not observed.CONCLUSION:EGF can induce proliferation and migration of NSCs during a special time(14-17 days).However,NSCs do not immigrate over the duration even they are caused by EGF.Migration has a transparent direction,I.e.,from big cell sphere to small one.