Wharton’s jelly mesenchymal stem cells differentiate into retinal progenitor cells*
Ying Hu
Jun Liang
Hongping Cui
Xinmei Wang
Hua Rong
Bin Shao
Hao Cui
摘要：Human Wharton’s jel y mesenchymal stem cel s were isolated from fetal umbilical cord. Cel s were cultured in serum-free neural stem cel-conditioned medium or neural stem cel-conditioned medium supplemented with Dkk-1, a Wnt/β catenin pathway antagonist, and LeftyA, a Nodal signaling pathway antagonist to induce differentiation into retinal progenitor cel s. Inverted microscopy showed that after induction, the spindle-shaped or fibroblast-like Wharton’s jel y mesenchymal stem cel s changed into bulbous cel s with numerous processes. Immunofluorescent cytochemical stain-ing and reverse-transcription PCR showed positive expression of retinal progenitor cel markers, Pax6 and Rx, as wel as weakly down-regulated nestin expression. These results demonstrate that Wharton’s jel y mesenchymal stem cel s are capable of differentiating into retinal progenitor cel s in vitro.
机标关键词：progenitor cellssignaling pathwayin vitro
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 1783-1792 )
英文信息