Morphology of platelet Golgi apparatus and their significance after acute cerebral infarction***
Wei Lu
Dong Xu
Ranran Tu
Zhiping Hu
摘要： Blood samples were harvested from the antecubital vein of 20 fasting patients with acute cerebral infarction at 1, 7 and 15 days after onset to prepare blood platelet suspension. Fasting antecubital vein blood was col ected from an additional 20 normal adults as controls. Under transmission tron microscope, platelet Golgi tubules and vesicles became significantly thickened, enlarged, and irregular after acute cerebral infarction. Alpha granules in platelets significantly reduced in number, especial y 1 day after cerebral infarction. Under immunoelectron microscopy, a few alpha granules aggregated around Golgi tubules and vesicles after infarction. These results suggested that platelet Golgi apparatus displayed significant morphological changes, which were possibly associated with enhanced synthetic and secretory functions of activated platelets after acute cerebral infarction. This study used Golgi apparatus blocking agent Brefeldin A to block Golgi apparatus in an aim to study the effects of Golgi apparatus on CD40L expression on the surface of activated platelets. Flow cytometry revealed that CD40L expression on activated platelet surfaces decreased significantly when Golgi apparatus was blocked, which indicated that Golgi apparatus participated in the syn-thesis and transport of CD40L to the platelet surface.
机标关键词：acute cerebral infarctionGolgi apparatusimmunoelectron microscopymorphological changesblood platelet
资助基金：grants from the National Natural Science Foundation of China, (No.81171239/H0914)the Education Expenditure of Hunan Provincial Finance Department in China, (No.2010163)
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 2134-2143 )
英文信息