Brain-derived neurotrophic factor inhibits glucose intolerance after cerebral ischemia***
Xiaoliang Shu
Yongsheng Zhang
Han Xu
Kai Kang
Donglian Cai
摘要：Brain-derived neurotrophic factor is associated with the insulin signaling pathway and glucose tabolism. We hypothesized that expression of brain-derived neurotrophic factor and its receptor may be involved in glucose intolerance fol owing ischemic stress. To verify this hypothesis, this study aimed to observe the changes in brain-derived neurotrophic factor and tyrosine kinase B receptor expression in glucose metabolism-associated regions fol owing cerebral ischemic stress in mice. At day 1 after middle cerebral artery occlusion, the expression levels of brain-derived neurotrophic factor were significantly decreased in the ischemic cortex, hypothalamus, liver, skeletal muscle, and pancreas. The expression levels of tyrosine kinase B receptor were decreased in the hypothalamus and liver, and increased in the skeletal muscle and pancreas, but remained unchanged in the cortex. Intrahypothalamic administration of brain-derived neurotrophic factor (40 ng) suppressed the de-crease in insulin receptor and tyrosine-phosphorylated insulin receptor expression in the liver and skeletal muscle, and inhibited the overexpression of gluconeogenesis-associated phosphoenolpy-ruvate carboxykinase and glucose-6-phosphatase in the liver of cerebral ischemic mice. However, serum insulin levels remained unchanged. Our experimental findings indicate that brain-derived neurotrophic factor can promote glucose metabolism, reduce gluconeogenesis, and decrease blood glucose levels after cerebral ischemic stress. The low expression of brain-derived neurotrophic factor fol owing cerebral ischemia may be involved in the development of glucose intolerance.
机标关键词：cerebral ischemianeurotrophic factorskeletal muscleglucose metabolisminsulin receptortyrosine kinasemiddle cerebral arteryblood glucose levels
资助基金：This study was supported by the Talent Foundation of the Affiliated Hospital of Guangxi Medical College in China, (No.08026)Youth Researcher Foundation of Guangxi Medical College in China, (No.08012)Scientific Research Foundation from Science and Technology Bureau of Shanghai City, (No.074119048)
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 2370-2378 )
英文信息