Baicalin inhibits colistin sulfate-induced apoptosis of PC12 cells******
Hong Jiang
Pengfei Lv
Jichang Li
Hongjun Wang
Tiezhong Zhou
Yingzi Liu
Wei Lin
摘要：Baicalin, a type of flavonoid extracted from the dried root of Scutel aria baicalensis georgi, has been shown to effectively inhibit cellapoptosis. Therefore, we assumed that baicalin would suppress colistin sulfate-induced neuronal apoptosis. PC12 cells exposed to colistin sulfate (62.5-500 μg/mL) for 24 hours resulted in PC12 cellapoptosis. In addition, caspase-3 activity, lactate dehydrogenase level and free radical content increased in a dose-dependent manner. Subsequently, PC12 cells were pretreated with baicalin (25, 50 and 100 μg/mL), and exposed to 125 μg/mL colistin sulfate. cellmorphology markedly changed, and cellviability increased. Moreover, caspase-3 activity, lac-tate dehydrogenase level and free radical content decreased. Results indicated that baicalin inhib-ited colistin sulfate-induced PC12 cellapoptosis by suppressing free radical injury, and reducing caspase-3 activity and lactate dehydrogenase activity.
机标关键词：free radicaldehydrogenase activityneuronal apoptosistype of
资助基金：The work was supported by the National Natural Science Foundation of China, (No.31201951 and 31272613)the Scientific and Technological Innovation Talent Scientific Research Foundation for the Returned Overseas Chinese Scholars by State Education Ministry and Heilongjiang Province in China, (No.2012RFLXN005 and LC201018)the Youth Science and Technology Foundation of Liaoning Medical University in China, (No. Y2012Z023)the Science and Technology Department of Liaoning Provincial Foundation Programs, (No.2011214001)
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 2597-2604 )
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