Occludin and connexin 43 expression contribute to the pathogenesis of traumatic brain edema*
Wanyin Ren
Guojie Jing
Qin Shen
Xiaoteng Yao
Yingchao Jing
Feng Lin
Weidong Pan
摘要：The experimental model of traumatic brain injury was established in Sprague-Dawley rats according to Feeney’s free fal ing method. The brains were harvested at 2, 6 and 24 hours, and at 3 and 5 days after injury. Changes in brain water content were determined using the wet and dry weights. Our results showed that water content of tissue significantly increased after traumatic brain injury, and reached minimum at 24 hours. Hematoxylin-eosin staining revealed pathological impairment of brain tissue at each time point after injury, particularly at 3 days, with nerve celledema, degenera-tion, and necrosis observed, and the apoptotic rate significantly increased. Immunohistochemistry and western blot analysis revealed that the expression of occludin at the injured site gradual y de-creased as injury time advanced and reached a minimum at 3 days after injury; the expression of connexin 43 gradual y increased as injury time advanced and reached a peak at 24 hours after in-jury. The experimental findings indicate that changes in occludin and connexin 43 expression were consistent with the development of brain edema, and may reflect the pathogenesis of brain injury.
机标关键词：traumatic brain injurywater contentinjury timewestern blot analysisexperimental modelbrain edematime point
资助基金：This study was supported by the Natural Science Foundation of Guangdong Province, (No.10151600101000002)
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 2703-2712 )
英文信息