Toll-like receptor 4-mediated signaling participates in apoptosis of hippocampal neurons**
Yue He
Ailing Zhou
Wei Jiang
摘要：The phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) signaling pathway is considered important for cellsurvival and has been shown to mediate various anti-apoptotic biological effects. This study explored the role of the Tol -like receptor 4 (TLR4)-mediated PI3K/AKT-glycogen syn-thase kinase 3β (GSK-3β) signaling pathways in lipopolysaccharide-induced apoptosis in a primary culture of hippocampal neurons. Results demonstrated that the apoptotic ratio of hippocampal neurons stimulated by lipopolysaccharide was significantly higher compared with the control group. Both the expression of P-AKTSer473 and P-GSK-3βSer9 in hippocampal neurons stimulated by lipopo-polysaccharide decreased compared with the control, while the level of active Caspase-3 and the ratio of Bax/Bcl-2 were significantly increased. The level of active Caspase-3 and the ratio of Bax/Bcl-2 in hippocampal neurons treated with TLR4 antibody or the GSK-3β inhibitor, LiCl, creased before intervention with lipopolysaccharide, but increased after treatment with the AKT hibitor, LY294002. These findings suggest that the TLR4-PI3K/AKT-GSK3β signaling pathway may be involved in lipopolysaccharide-induced apoptosis of hippocampal neurons.
机标关键词：hippocampal neuronsprimary culture
资助基金：This study was supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions and Graduate Scientific and Technological Innovation Projects of Nantong University, (No. YKC12020)Applied Research and Technology Plan of Nantong City, (No. BK2013007)
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 2744-2753 )
英文信息