Normobaric oxygen for cerebral ischemic injury
Chunhua Chen
Haimeng Cui
Zihe Li
Ruifeng Wang
Changman Zhou
摘要：Oxygen inhalation has been shown to increase oxygen supply to tissues after cerebral ischemia/reperfusion injury, protecting injured neural cells. However, hyperbaric oxygen may aggravate oxidative stress. By contrast, normobaric oxygen has the rapid and non-invasive characteristics and may have therapeutic effects on ischemic/hypoxic disease. Rats inhaled normobaric oxygen (95%O 2 ) for 6 consecutive days, and then a rat model of focal cerebral ischemia was established. Nissl and 2,3,5-triphenyltetrazolium chloride (TTC) staining revealed that normobaric oxygen pretreat-ment improved neurological deficits and reduced infarct volume. Immunohistochemical staining and western blot assay revealed that the expression of hypoxia-inducible factor-1α, Notch-1, vascular endothelial growth factor and erythropoietin were increased. Behavioral studies also verified that neurological deficit scores increased. The hypoxia-inducible factor inhibitor 2-methoxyestradiol treatment at 1 hour before administration of normobaric oxygen could suppress the protective effect of normobaric oxygen. Given these observations, normobaric oxygen pretreatment may al eviate cerebral ischemic injury via the hypoxia-inducible factor signal pathway.
机标关键词：focal cerebral ischemiaendothelial growth factorneurological deficitstherapeutic effectsprotective effectoxidative stresssignal pathwayoxygen supply
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 2885-2894 )
英文信息